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Objective:To analyze the effect of cognitive conflict in teaching practice nurses in emergency department.Methods:102 nursing students who practiced in the emergency department of Huai′an First Hospital Affiliated to Nanjing Medical University were selected as the research objects by quasi-experimental study, 52 students from December 2021 to July 2022 were set as the research group, and 50 students from March 2021 to November 2021 were set as the control group. The control group received on-site first aid sitnation teaching. The research group combined cognitive conflict teaching on the basis of on-site first aid situation teaching, and compared the emergency nursing skills, theoretical knowledge, critical thinking, clinical strain ability and teaching satisfaction of the two groups of interns.Results:After practice, the scores of emergency nursing skills, theoretical knowledge, critical thinking ability, clinical adaptability and satisfaction with the teaching mode (theoretical memory, interest in learning, practical ability) in the research group were (87.25 ± 2.66), (90.01 ± 2.74), (298.10 ± 8.27), (84.37 ± 3.45), (90.13 ± 3.26), (89.03 ± 3.37) and (91.34 ± 3.16), which were significant higher than the (83.76 ± 2.87), (85.16 ± 3.11), (290.47 ± 8.55), (80.05 ± 3.68), (85.46 ± 3.53), (86.28 ± 3.61) and (88.47 ± 3.38), the difference were significant ( t values were 3.98-8.37, all P<0.05). Conclusions:Cognitive conflict teaching can significantly improve the professional level, critical thinking and clinical adaptability of nursing interns and their satisfaction with teaching.
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Glaucoma is one of the most common optic neuropathies, featuring progressive retinal ganglion cell damage and visual field loss (Tham et al., 2014; Xu et al., 2020). Currently, the only effective treatment for this condition is the reduction of intraocular pressure (IOP) (Palmberg, 2001; Heijl et al., 2002). Canaloplasty is a proven bleb-independent surgery with good efficacy and safety profiles in primary open-angle glaucoma (POAG) (Gołaszewska et al., 2021). However, early transient postoperative IOP elevation has been reported in up to 30% of cases (Riva et al., 2019), similar to that commonly observed in other internal drainage glaucoma surgeries such as implantation using iStent (0%-21.0%), CyPass (10.8%), and Hydrus (4.8%-6.5%) (Lavia et al., 2017). This complication may be a predictor of poor reserve in the outflow system and is potentially associated with surgical failure. Nonetheless, the exact pathophysiology of glaucoma remains unknown, and studies clarifying the risk factors for postoperative IOP elevation have been scarce.
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Humans , Intraocular Pressure , Glaucoma, Open-Angle/surgery , Incidence , Treatment Outcome , Risk FactorsABSTRACT
Creeping hair is a rare condition characterized by creeping eruption with a black line at the advancing end, mimicking cutaneous larva migrans. The condition is also referred to as cutaneous pili migrans, migrating hair, and embedded hair. A total of 52 cases have been reported since 1957 and most cases were published in English. Herein, we report a case in which creeping hair occurred in the iliac region and review the literature from 1957 to February 2021. A 35-year-old Chinese female presented with a black moving linear eruption that had migrated from the lower abdomen to the iliac region without causing any symptoms during a 3-year period. Cutaneous examination showed a 6.5-cm-long black linear lesion beneath the skin that was revealed to be a hair shaft. After removal of the hair, the eruption diminished and no recurrence occurred in 3 months of follow-up. The creeping hair that had migrated with its lower end forward was confirmed by observation under dematoscopy and light microscopy. A review of the literature revealed that creeping hair occurs most frequently in young and middle-aged patients and the reported cases are mainly from Asia. The top locations involve the foot. The causative hair includes head hair, beard, pubic hair, body hair, and one case of dog hair. A closeup examination and dermoscopic inspection are helpful for the diagnosis of creeping hair.
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Objective:To investigate the per capita expenses of physical examination and its related factors in health examination (management) institutions in 2019.Methods:Using the census method, the basic information, including the name, address, nature, level and operation nature of the institutions, and per capita expenses of physical examination in provincial health examination (management) institutions in 2019 were collected by questionnaire survey. Rank sum test and Spearman correlation analysis were used to analyze the data.Results:In 2019, the top three provinces in terms of per capita expenses of physical examination were Shanghai, Inner Mongolia Autonomous Region and Beijing, with 612.00 yuan, 547.90 yuan and 528.83 yuan, respectively; Guizhou Province, Xinjiang Uygur Autonomous Region and Guangdong Province ranked the last three, with 255.25 yuan, 244.00 yuan and 235.10 yuan, respectively. There were significant differences in per capita expenses of physical examination among different health examination (management) institutions in different regions and of different institutional nature, institutional level and business nature (all P<0.05). The per capita expenses of physical examination was positively correlated with the local per capita health care expenditure, the proportion of local population aged 15-64 years and the proportion of local people with education of college and above ( r=0.465, 0.374 and 0.401, all P<0.05). Conclusions:There are differences in the per capita expenses of physical examination in health examination (management) institutions among different regions, institutional nature, institutional level and business nature in 2019, and it is related to the per capita health care expenditure, age and educational level of the local people.
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【Objective】 To explore the challenging blood cross-matching and resolution for multiple myeloma (MM) patients in different disease stages. 【Methods】 For a patient who was first diagnosed as MM and scheduled for blood transfusion, his blood was cross matched with donors’ blood by microcolumn gel method and tube test. When the major side of cross-matching was agglutinated, the patient’s plasma was cross matched with donors’ red blood cell (RBC) by polybrene test, then plasma dilution cross matched with donors’ RBC by microcolumn gel method. For a patient who was diagnosed as recurrent refractory MM and scheduled for blood transfusion, his blood was cross matched with donors’ blood by microcolumn gel method. 【Results】 1) Case 1 was a first-visit outpatient. The major side of microcolumn cross-match test was agglutinated with the shape of fine line. The result of tube method also showed agglutination of major sides, and the rouleaux were detected by the microscopy. Then polybrene method and microcolumn gel method (after plasma diluted) were applied for cross-matching again with the above two donors’ blood and showed compatibility. 2) Case 2 was a recurrent refractory MM patient. The major and minor sides of microcolumn cross-match test were both agglutinated with the shape of granular. The patient was treated with anti-CD38 monoclonal antibody. The RBCs, after treated with dithiothreitol (DTT) was used to cross match with patient plasma by microcolumn test, and the result was compatible. 【Conclusion】 Polybrene method and microcolumn gel method after plasma diluted are suitable for blood cross-matching of newly diagnosed MM patients, also for those treated with CD38 monoclonal antibody, as the drug interference with cross-matching can be eliminated by DTT.
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Objective:To understand the screening of cervical cancer in our county and provide epidemiological data for the prevention and control of cervical cancer.Methods:A total of 3 034 healthy women with 30~65 years old in Chun'an county were recruited in this prospective study from June 2015 to January 2016.All of the subjects received cervical cancer screening.The respondents were randomly divided into non-typeable HPV screening group (2018 cases) and the vision inspection with acetic acid and visual inspection with Lugol's iodine screen (VIA/VILI) group (1016 cases). Direct colposcopy or VIA/VILI visual examination was performed for those with positive HPV, and then colposcopy was performed for those with positive visual examination.The suspicious lesions tissues were applied for pathology detection.From September 2017 to February 2018, 2 363 patients who were initially negative received VIA/VILI combined with unclassified HPV examination, and those who were positive received colposcopy and pathological biopsy.The positive rate of primary screening and cervical squamous intraepithelial neoplasia disorders level 2 and above (CIN Ⅱ + ) lesion detection rate were analyzed in the two-stage screening.Results:From June 2015 to January 2016, the preliminary screening positive rate of unclassified HPV group was 11.60% (234/2 018). A total of 150 patients with positive results were recalled for colposcopy, with a recall rate of 64.10% (150/234). Among the 150 cases, 119 cases (79.33%) were positive in the unclassified HPV preliminary screening, and 31 cases (20.66%) were positive in VIA/VILI examination after HPV preliminary screening.The positive rate of VIA/VILI initial screening group was 20.28% (206/1 016), and the recall rate of colposcopy was 99.51% (205/206). A total of 108 cases underwent colposcopy with abnormal pathological biopsy examination in the two groups, and the pathological biopsy rate was 30.34% (108/356), and the pathologic biopsy CIN Ⅱ+ lesion detection rate was 297/100 000 (9/3 034). In the second round of screening from September 2017 to February 2018, 2 363 subjects were recruited back to the baseline, with a recall rate of 77.89% (2 363/3 034). In the 2 363 cases, the early screen VIA/VILI anomalies or HPV positive early screening positive rate was 17.01% (402/2 363), and the colposcopy examination the recall rate was 80.10% (322/402), and 80 cases received vaginal lens suspicious pathological biopsy, the pathological biopsy rate was 24.84% (80/322), the pathological results showed that CIN Ⅱ+ detection rate of 296/100 000 (7/2 363).Conclusion:The detection rate of precancerous suspicious lesions in cervical cancer screening in our county is lower than the national average level.On the one hand, we should strengthen the propaganda and education of active screening, improve the compliance and cooperation degree of screening objects, and improve the recall rate of suspicious cases.On the other hand, the government should invest more efforts to select the screening technology with high sensitivity, improve the screening technology of doctors at the basic screening point, and improve the detection rate of cervical cancer prelesions.
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Objective:To explore the application value of dynamic monitoring of serum soluble interleukin-2 receptor (sIL-2R) and stromal cell-derived factor 1 α (SDF-1α) in patients with acute leukemia (AL).Methods:A total of 187 patients with AL admitted to the Second Hospital of Shanxi Medical University from March 2017 to August 2018 were selected and 90 healthy subjects at the same period were selected as the healthy controls. The levels of serum sIL-2R and SDF-1α were detected at the initial diagnosis, remission phase and relapse phase, respectively. The clinical value of dynamic monitoring of sIL-2R and SDF-1α was analyzed.Results:Among 187 patients with AL, 135 patients (72.19%) had complete remission (CR) after chemotherapy, 52 patients (27.81%) had partial remission, and 43 patients (31.85%) relapsed. The level of serum sIL-2R at the AL initial diagnosis period was (533±32) U/ml, which was higher than that in the healthy controls [(247±30) U/ml], and the difference was statistically significant ( t = 71.976, P < 0.01); the level of serum SDF-1α at the AL initial diagnosis was (2 968±305) pg/ml, which was higher than that in the healthy controls [(1 358±160) pg/ml], and the difference was statistically significant ( t = 47.043, P < 0.01). The levels of serum sIL-2R [(308±30) U/ml] and SDF-1α [(1 576±184) pg/ml] in the CR phase were lower than those in the initial diagnosis of patients with AL; and the levels of sIL-2R [(599±36) U/ml] and SDF-1α [(2 894±301) pg/ml] in the relapse phase were higher than those in the CR phase of patients with AL (all P < 0.01). Conclusions:The levels of serum sIL-2R and SDF-1α in patients with AL are increased, and there are big differences in the levels of sIL-2R and SDF-1α at the initial diagnosis, remission phase, and relapse phase. Dynamic monitoring of both can provide the data support for early clinical intervention.
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Objective To evaluate the clinical value of non-invasive skin autofluorescence measurement of advanced glycation end products (AGE) to screen for both diabetes mellitus and impaired glucose tolerance (IGT). Methods A total of 389 individuals who underwent physical examination at Renmin Hospital of Wuhan University from December 2016 to August 2017, and were willing to participate were enrolled in our study. 293 people meeting the criteria were included finally. AGE value in the skin tissue was measured non-invasively via autofluorescence with the DM Scanner. Two-hour oral glucose tolerance testing, and measurements of fasting blood glucose and glycosylated hemoglobin were also performed for each patient. Results Among 293 subjects, 209 had normal glucose metabolism, 50 had IGT, and 34 had diabetes mellitus. The measured Advanced glycation end product values in these groups were (69.44 ± 7.28) AU, (79.67 ± 5.65) AU, and (81.17 ± 9.28) AU, respectively. The differences between groups were statistically significant (P<0.05). Pearson correlation analysis showed a significant positive correlation between autofluorescence measurements and fasting blood glucose, glycosylated hemoglobin and glucose level 2 hours after oral glucose loading (r=0.472, 0.433, and 0.546, respectively;P<0.001). The sensitivity of DM Scan screening for IGT was 84.00％, with a specificity of 84.87％. For diabetes mellitus, the sensitivity was 79.41％, and the specificity 84.87％. Conclusion DM Scan has high sensitivity in screening for diabetes mellitus and IGT. It has important clinical value in the early diagnosis of IGT.
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An important and unresolved question is how human brain regions process information and interact with each other in intertemporal choice related to gains and losses. Using psychophysiological interaction and dynamic causal modeling analyses, we investigated the functional interactions between regions involved in the decision-making process while participants performed temporal discounting tasks in both the gains and losses domains. We found two distinct intrinsic valuation systems underlying temporal discounting in the gains and losses domains: gains were specifically evaluated in the medial regions, including the medial prefrontal and orbitofrontal cortices, and losses were evaluated in the lateral dorsolateral prefrontal cortex. In addition, immediate reward or punishment was found to modulate the functional interactions between the dorsolateral prefrontal cortex and distinct regions in both the gains and losses domains: in the gains domain, the mesolimbic regions; in the losses domain, the medial prefrontal cortex, anterior cingulate cortex, and insula. These findings suggest that intertemporal choice of gains and losses might involve distinct valuation systems, and more importantly, separate neural interactions may implement the intertemporal choices of gains and losses. These findings may provide a new biological perspective for understanding the neural mechanisms underlying intertemporal choice of gains and losses.
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Adult , Female , Humans , Male , Young Adult , Brain , Diagnostic Imaging , Physiology , Brain Mapping , Delay Discounting , Physiology , Magnetic Resonance Imaging , Neural Pathways , Diagnostic Imaging , Physiology , Neuropsychological Tests , Psychophysics , RewardABSTRACT
Objective · To explore the correlation between variants located in 3' untranslated regions (3'UTR) of NOTCH1 and JAG1 genes and conotruncal heart defects (CTD). Methods · Six hundred CTD children without 22q11 deletion and three hundred healthy children were enrolled in this hospital-based case-control study. Variants located in the 3'UTR regions of NOTCH1 and JAG1 genes were detected by high-throughput sequencing. The accuracy of the variants were verified by PCR and Sanger sequencing. Online software PicTar, TargetScan and microRNA.org were used to make functional predictions. Results · One mutation and three SNPs were found in the 3'UTR of NOTCH1. Three mutations and six SNPs were found in the 3'UTR of JAG1. The genotypic distributions of two SNPs (rs3840074 and rs8708) located in JAG13'UTR between CTD group and the controls were statistically significant (both P<0.05). Results of prediction showed that all the four mutations and two meaningful SNPs could bind to microRNA. Conclusion · The variants located in 3'UTR regions of NOTCH1 and JAG1 genes may be related to the occurrence of CTD.
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Objective To investigate the Toxoplasma gondii infection status in pregnant women with history of adverse preg?nancy and risk factors in Bazhou area,Hebei Province. Methods A total of 302 pregnant women with the history of adverse pregnancy were chosen as respondents(an experimental group)in the hospital from March 2012 to December 2015,and 197 pregnant women without the history of adverse pregnancy as a control group. TOX?IgG and TOX?IgM were detected by using ELI?SA in two groups. The risk factors of Toxoplasma infection were surveyed by questionnaires. Results The total positive rate of Toxoplasma antibodies was 28.15%(85/302)in the experimental group,which was significantly higher than that[9.64%(19/197)]in the control group,and the difference was statistically significant(χ2=24.76,P<0.05). The positive rates of TOX?IgM,TOX?IgG and TOX?IgM+TOX?IgG were 6.95%(21/302),18.54%(56/302),and 2.65%(8/302)respectively in the ex?perimental group,which were higher than 2.03%(4/197),7.61%(15/197),and 0%(0/197)respectively in the control group (χ2=6.07,11.67,3.76,all P<0.05). The questionnaire survey showed that the proportions of keeping pets,cutting board re?gardless,liking to eat hot pot or barbecue,eating raw meat,often eating in the restaurant in the pregnant women with Toxoplas?ma infection were higher than those in the pregnant women without Toxoplasma infection,and the differences were statistically significant(χ2=22.57,3.96,5.87,7.40,4.86,all P<0.05),and therefore,the above unhealthy habits may be important risk factors. Conclusions Toxoplasma infection could lead to adverse pregnancy outcomes. Therefore,the above?mentioned unhealthy habits should be avoided,especially during pregnancy period.
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Objective To explore the relationship between depression and anxiety and peripheral blood cell counts ( white blood cell ( WBC) ,neutrophil ( NEU) ,lymphocyte ( LYM) ,red blood cell ( RBC) and hemoglobin ( HGB) ) of heroin?dependent patients receiving methadone maintenance treatment ( MMT) . Methods Two hundred and fifty?two MMT heroin addicts, who had received peripheral blood tests within the week prior to the study,were administered with a general information questionnaire,SDS and SAS. The results of blood tests of these patients were compared with those of 109 healthy people in physical examina?tion. Multiple linear regression was used to analyze associations between indicators of peripheral blood cell counts and depressive and anxiety symptoms. Results Compared with healthy controls,MMT patients had significantly higher proportions of elevated WBC(14.7%vs 3.7%),NEU(12.7% vs 2.8%) and LYM(9.9%vs 2.8%) counts (all P<0.05),and low RBC(10.3%vs 4.6%) counts and HGB(11.5%vs 4.6%) concentra?tion (both P<0.05). Multivariate linear regression analysis showed that blood cell counts of WBC ( β=0.261, P<0.01),NEU ( β=0.264, P<0.01),LYM ( β=0.196, P=0.003) and RBC ( β=-0.144, P=0.021) were independently associated with depression;and blood cell counts of WBC ( β=0.298, P<0.01) and LYM ( β=0.269, P<0.01) were independently associated with anxiety. Conclusion Improving the in?flammatory and malnutrition status may be helpful for reducing depressive and anxiety symptoms of MMT her?oin addicts.
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Objective To investigate the status and difference of learning burnout between Korean-Chinese nursing students and Chinese nursing students;and to compare the influencing factors of learning burnout between them.Methods A total of 307 nursing students in Yanbian University were recruited by convenience samphng method.Data were analyzed by SPSS 20.0,using descriptive statistics,t-test,ANOVA,correlation analysis,and linear regression analysis.Results The emotion and learning burnout of Korean-Chinese nursing students scored (3.18±0.52)points and (2.84±0.33)points,which were higher than (3.04±0.53)points and (2.69±0.36) points of Chinese nursing students (t=5.72,4.19,all P ＜ 0.05);The significant predictors of learning burnout of Korean-Chinese nursing students were effective commitment,continuance commitment and ideality commitment;and the significant predictors of Chinese nursing students were effective commitment,continuance commitment and behavior self-efficacy.Conclusions Because of the special background of Korean-Chinese nursing students,it is suggested payed attention to improve their major commitment and learning self-efficacy,then to decrease their learning burnout.
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Emerging evidence has indicated that BRCC 36-mediated K63-linked ubiquitination modification was involved in diverse cellular functions , including endocytosis , apoptosis and DNA damage repair .We previously showed that activation of cGMP/PKG pathway con-tributed to the binding of BRCC36 and the pro-fibrotic factor Smad3.The current study tested the hypothesis that BRCC 36 functions as a negative regulator of transforming growth factor-beta ( TGF-β)/Smad3 pathway and participates in cardiac remodeling .In isolated adult mouse cardiac fibroblasts , we have demonstrated that TGF-β1 treatment significantly increased the expression of BRCC 36.Over-expression BRCC36 suppressed TGF-β1-induced Smad3 phosphorylation, nuclear translocation, extracellular matrix molecular expres-sion and cell proliferation .On the contrary, silencing BRCC36 by transfection of adenovirus-carrying BRCC36 shRNA potentiated to enhance the pro-fibrotic effect of TGF-β.In vivo, under chronic pressure overload condition-induced by transverse aortic constriction , myocardial pro-survival protein Bcl-2 and Mcl-1 expression were significantly decreased and the pro-apoptosis protein Puma was in-creased.However, the cardiac-specific over-expression of BRCC36 significantly increased myocardial Bcl-2 and Mcl-1 and inhibited Puma expression .Interestingly , we also found that sustained pressure overload resulted in a significant myocardial DNA injury in wild type mice, which was characterized by the increase of γH2AX level.However, cardiac-specific BRCC36 over-expression significantly decreased the level of γH2AX in the pressure overloaded heart in the transgenic mice , while effectively enhanced myocardial RAD 51 expression, a marker of DNA damage repair.Furthermore, BRCC36 over-expression effectively attenuated TAC-induced cardiac fibro-sis and remodeling in the transgenic mice , compared with the wild type mice .Collectively , the results have suggested that BRCC 36 ef-fectively protected heart against chronic pressure overload-induced cardiac remodeling though antagonizing TGF-β/Smad3 pathway and enhancing myocardial DNA injury repair response .
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Objective: The pathology workflow statistics, modeling, optimize procedure and establish standardized operating procedures. Methods:This paper which based on the previous studies, the standard operation management and database technology, based on pathology workflow sample collection, sample inspection, warehousing storage, picking or sorting, sample processing and handling, inspection, quality control and other aspects of the analysis, optimizing operating procedures, thus forming SOPs, and accordingly the establishment of standardized management database system. Results: The system shortens the pathological examination cycle, improve the detection efficiency. Conclusion: It can be widely used in pathology sample collection, diagnosis, management processes, and application for sound management system pathology, pathology strengthen quality control is important.
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<p><b>OBJECTIVE</b>To explore the effects of uterine adenomyosis on the clinical outcomes of infertility patients treated with in vitro fertilization/intracytoplasmic sperm injection-embryo transfer (IVF/ICSI-ET).</p><p><b>METHODS</b>A retrospective study was conducted of 61 IVF/ICSI-ET cycles as the study group, diagnosed with uterine adenomyosis by transvaginal ultrasound, and 164 IVF/ICSI-ET cycles of patients with tubal infertility as the control group. The baseline characteristics, ovary response and clinical outcomes were compared between the two groups.</p><p><b>RESULTS</b>The implantation rate, clinical pregnancy rate and live birth rate decreased significantly in the study group (P<0.05), and early abortion rate increased significantly (P<0.05). For patients with adenomyosis, GnRH-antagonist cycles tended to decrease clinical pregnancy rate and increase abortion rate (25.0% vs 45.0%, P=0.184; 66.7% vs 27.8%, P=0.247), and significantly decrease live birth rate (0% vs 30.8%, P=0.025), compared with GnRHa agonist cycles.</p><p><b>CONCLUSION</b>Uterine adenomyosis decreases implantation rate, clinical pregnancy rate and birth rate, and increases abortion rate significantly in patients with IVF/ICSI-ET. GnRH-antagonist cycles have adverse effects on the outcomes of adenomyosis; GnRH agonist long protocol cycles may increase clinical pregnancy rate and decrease abortion rate.</p>
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Female , Humans , Pregnancy , Adenomyosis , Embryo Implantation , Embryo Transfer , Fertilization in Vitro , Gonadotropin-Releasing Hormone , Hormone Antagonists , Infertility, Female , Pregnancy Complications , Pregnancy Rate , Retrospective Studies , Sperm Injections, IntracytoplasmicABSTRACT
Objective To construct and identify lentiviral vector pGC-FU-CXCR4 gene. Methods CXCR4 gene amplification was used by real-time polymerase chain reaction. The target gene fragments with the digested plasmids were exchange. Then the lentiviral vector pGC-FU-CXCR4 was constructed successfully. Use the constructed lentiviral vector to infect the competent escherichia coli cells. Polymerase chain reaction analysis was used to identify the cultural clones and DNA sequencing and comparative analysis were used to positive fragments. The successfully constructed plasmids had the same sequence with the target gene. Results Polymerase chain reaction tests showed that am-plified target genes were inserted in pGC-FU vectors. The electrophoresis results,digestion showed that the reconstructed plasmid was consist-ent with the theoretical fragment and the sequence result of the positive fragments were exactly the same with the target gene. Conclusion Lentiviral vectors of CXCR4 gene over-expression were successfully constructed.
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Salmonella enterica serovar Choleraesuis strain C500 is a live attenuated vaccine that has been widely used in Chi‐na for over 50 years to prevent piglet paratyphoid .However ,as C500 is obtained by chemical methods ,the genetic background of this strain remained unclear .In this study ,we compared the genomic differences between the virulent reference strain C 78‐2 and C500 by suppression subtractive hybridization combined with the mirror orientation selection method (MOS‐SSH ) .Six genes (asr ,ydgF ,ydgD ,ydgE ,rpoS ,and ptsG) were lost in C500 strain .Using real‐time PCR analysis ,we demonstrated that the genes regulated by rpoS ,a vital transcriptional regulator playing an important role in Salmonella infection ,were downregulated in C500 .Additionally ,the virulence of the rpoS mutant strain C78‐2ΔrpoS was 100 000 times lower than the parental strain in BALB/c mice .So loss of rpoS gene is the major factor leading to the attenuation of C500 strain .
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Objective To study the nutritional risks in elderly patients with prostate cancer and explore its related factors.Methods 346 elderly patients with prostate cancer in Wuhan area from May 2013 to May 2014 were chosen as the objects in this study.Nutritional risk screening 2002 was used to evaluate nutritional risk.The sleep quality,anxiety,lower urinary tract symptoms,differentiation and other data of patients were collected.The influencing factors for nutritional risk in the patients were analyzed.Results The ratio of nutritional risk in elderly advanced prostate cancer patients was 35.3％ (122/346).The nutritional risk was increased with age (P＜0.05).The prostate cancer patients with nutritional risk had a higher international prostate symptom score (IPSS) (P＜ 0.05).Monovariate factor analysis showed that sleep quality,operation,pathology staging,radiotherapy,chemotherapy,lower urinary tract symptoms (LUTS) were the impact factors for nutritional status in elderly patients with prostate cancer (P＜0.05).Logistic regression analysis showed that age (OR=0.29),sleep quality (OR=0.25) were the protective factors for the nutritional status,while surgery (OR=12.67),pathological staging (OR=1.65),radiotherapy (OR=2.65),SPSS (OR=1.55),chemotherapy (OR=1.85) were the risk factors for nutritional status (P＜ 0.05).Conclusions The incidence of nutritional risk is high in elderly prostate cancer patients.Age,sleep quality are the protective factors,and operation,pathology staging,SPSS,chemotherapy,and radiotherapy are the risk factors for nutritional status.
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We report 3 cases of successful pregnancies in women with a history of surgeries for gynecological malignancies and postoperative infertility, achieved by in vitro fertilization-embryo transfer (IVF-ET) with controlled ovarian hyperstimulation. All the 3 patients had clinical pregnancies without cancer recurrence. In such cancer survivors with infertility, the ovarian reserve is severely impaired by cancer therapies and assisted reproductive techniques should be the primary option.